Gel chromatographic separation of oligosaccharides at elevated temperature
نویسندگان
چکیده
منابع مشابه
Separation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis.
Glycosaminoglycan oligosaccharides generated by treatment of biosynthetically radiolabelled dermatan sulphate and hyaluronic acid with chondroitin AC lyase or testicular hyaluronidase may be resolved into a series of discrete bands by polyacrylamide-gel electrophoresis. Bands were identified by fixation in glacial acetic acid containing 20% (w/v) 2,5-diphenyloxazole followed by fluorography. Th...
متن کاملEffect of temperature on symmetry breaking excited state charge separation: restoration of symmetry at elevated temperature.
With an increase in temperature, an unprecedented restoration of symmetry in the symmetry breaking excited state charge transfer is observed in a geminal pair of near-orthogonally connected perylenimide dimers. Such restoration of symmetry could be attributed to the interchromophoric planarization and/or loss of solvation asymmetry at elevated temperature resulting in enhanced fluorescence quan...
متن کاملHigh-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrosp...
متن کاملChromatographic separation of glycated nucleotides.
A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...
متن کاملChromatographic Separation of the Diastereoisomers
Reduction of folic acid to the tetrahydro level introduces a second asymmetrical center into the molecule at carbon atom 6.1 The resulting tetrahydrofolate, and subsequent derivatives, prepared chemically consist of dl mixtures that are only 50% active in biological reactions. The need for fully reactive isomers of reduced folates led to the enzymatic synthesis of tetrahydrofolate from 7, %dihy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1968
ISSN: 0014-5793
DOI: 10.1016/0014-5793(68)80104-8